| 摘要 |
The invention provides compositions and methods for mass spectrometric (MS), organic synthesis, and applications of organo-trifluoroborate, for example, as mass tags for use in negative ion mode. When subject to MS fragmentation, organo-trifluoroborates preferentially undergo neutral losses of hydrogen fluoride (HF) or boron trifluoride (BF3) molecules, transferring the negative charge to the rest of the molecule. Such a fragmentation pattern is used to detect and quantitate analytes of interest after derivatization with organo-trifluoroborates. |
| 主权项 |
1. An organo-bis-trifluoroborate compound having structural formula: wherein Y2 is a functional group selected from the group consisting of an alcohol, alkoxy, aryloxy, diene, dienophile, carboxylic acid, amine, thiol, alkyl halide, ketone, aldehyde, aminooxy, hydrazine and its derivatives, activated ester, acid halide, isocyanate or thio-isocyanate, azo, alkyne, 1,3-dipolariphile, sulfonyl chloride, glyoxal, epoxide or oxirane, carbonate, aryl halide such as fluorobenzene derivatives, imidoester or imidate, anhydride, fluorophenyl ester, hydroxymethyl phosphine derivative, maleimide, aziridine, acryloyl derivative, arylating agent such as derviatives of benzene that possess either halogen or sulfonate groups on the ring, thiol-disulfide exchange reagent such as pyridyldithiol and thiolnitrobenzoic acid, or vinylsulfone group; L2 is a linker or a combination of 2 to 4 linkers that are independently selected from the group consisting of C1-C20 alkyl, —(CH2CH2O)1-36—, —(CH2CH2O)1-36—CH2—, a peptide, a peptidomimetic oligomer, alicyclyl, optionally substituted alicyclyl, heteroalicyclyl, optionally substituted heteroalicyclyl, and optionally substituted aryl; and X⊕ is any positively charged counter ion. |
