| 摘要 |
<p>PURPOSE:To prepare a solution for refrigerating mammalian cells, which contains a specific protein such as keratin hydrolyzate and is useful for refrigeration of hepatic cells or the like because it gives good survival rate during the refrigeration. CONSTITUTION:Dimethyl sulfoxide(DMSO) is added to the base medium for the cell culture, further keratin hydrolyzate, gelatin hydrolyzate or serum albumen is added. The hydrolyzed keratin or the gelatin hydrolyzate is added in an amount of more than 0.5wt.%, preferably 5 to 15wt.%, while serum albumen is added in an amount of more than 0.5wt.%, preferably more than 2.5wt.%. The survival rate of the cells after freezing and thawing is more improved than in using a storage solution containing only DMSO or DMSO and serum.</p> |
