发明名称 |
Method and system for detecting inter-chromosomal imbalance by fluorescent in situ hybridization (fish) on interphase nuclei |
摘要 |
The invention concerns a system for detecting chromosomal imbalance by in situ hybridization of fluorescent probes on interphase nuclei, comprising the following phases: hybridizing in situ fluorescent probes on two separate chromosomes; exposing each probe with a different fluorochrome; measuring the intensity signals corresponding respectively to each probe thus exposed, on an assembly of nuclei, said measurement being carried out within a control cell population and within a cell population subjected to detection; calculating a ratio between the signals corresponding to each of the probes, said ratio calculation being carried out on said control cell population to provide a reference ratio and said population subjected to detection; comparing the fluorescence ratio corresponding to the population subjected to detection with the reference ratio; and processing the result of said comparison to detect an inter-chromosomal imbalance.
|
申请公布号 |
US2005042609(A1) |
申请公布日期 |
2005.02.24 |
申请号 |
US20040482809 |
申请日期 |
2004.10.06 |
申请人 |
(1) IMSTAR IMAGE ET MODELISATION: STRATEGIE ANALYSE ET REALISATION (2) INSTITUT CURIE (ET AL) |
发明人 |
SOUSSALINE FRANCOISE;MALFOY BERNARD;GUILLY MARIE NOELLE;TRUONG KHUONG;DUTRILLAUX BERNARD |
分类号 |
G01N21/64;C12N15/09;C12Q1/02;C12Q1/68;G01N21/78;G01N33/483;G01N33/53;G01N33/566;(IPC1-7):C12Q1/68;C07H21/04 |
主分类号 |
G01N21/64 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|