发明名称 |
Solid substrate comprising array of dendrons and methods for using the same |
摘要 |
The present invention provides solid supports comprising a surface bound array of dendrons and methods for using the same. |
申请公布号 |
US9389227(B2) |
申请公布日期 |
2016.07.12 |
申请号 |
US200812102794 |
申请日期 |
2008.04.14 |
申请人 |
POSCO;POSTECH FOUNDATION |
发明人 |
Park Joon Won;Hong Bong Jin |
分类号 |
C12M1/34;C12M1/00;C12Q1/68;C07H21/02;C07H21/04;C40B30/04;G01N33/543;C40B50/18 |
主分类号 |
C12M1/34 |
代理机构 |
Hamilton DeSanctis & Cha, LLP |
代理人 |
Cha Don D.;Hamilton DeSanctis & Cha, LLP |
主权项 |
1. An array of dendrons, said array comprising a solid support having at least a first surface; and a plurality of conically shaped dendrons covalently attached to said first surface of said solid support, wherein each of said dendron is of the formula:
Z—R1-Q1-{(R3-Q3)b-[(R4-Q4)c-(R5—Y)x]y}3 wherein
c is 0 or 1; x is 1 when c is 0 or when c is 1, x is 3; y is 1 when b is 0 or when b is 1, y is 3; Z is X-O-C(═O)—NH—, wherein X is a nucleic acid probe; R1 is —(CH2)3—C(═O)—NH—; Q1, Q3 and Q4 are C; R3 is —CH2—O—(CH2)2—C(═O)—NH—(CH2)3—C(═O)—NH—; R4 is —CH2—O—(CH2)2—C(═O)—NH—; R5 is —CH2—O—(CH2)2—; Y is a terminal functional group moiety of the formula —COOH;wherein a plurality of said terminal functional groups on each of said dendron are covalently attached to said first surface of said solid support, and wherein said dendrons are spaced at regular intervals between about 0.1 nm and about 100 nm, and wherein a discrimination efficiency of the probe is at least 80%. |
地址 |
Pohang-Si, Kyungsangbuk-Do KR |