Instrument and method for detecting analytes

摘要

The present disclosure provides instruments and methods for detecting an analyte which are capable of exciting a plurality of luminescence labels and detecting light emitted therefrom. The instrument includes a filter carrier adapted for carrying a plurality of filter portion pairs, each pair related to a luminescence label and comprising a first filter portion for transmitting excitation light, and a second filter portion for transmitting emitted light. The first filter portion of a pair comprises a second filter portion of another pair. Also, the filter portions are arranged such that a pair can be brought into an operative condition whereby a first filter portion is in the excitation beam path and a second filter portion is in the emission beam path. The filter carrier and beam paths may be moved with respect to each other by a moving mechanism so as to bring a pair into operative condition.

主权项

1. An instrument for detecting an emission light emitted from one or more of a plurality of luminescence labels associated with an analyte in a sample, comprising:
a mount configured to support a vessel for housing one or more samples; a light source capable of generating an excitation light for exciting said plurality of luminescence labels associated with said analyte in one or more samples housed in said vessel; an excitation arrangement configured to propagate said excitation light along an excitation beam path positioned between said light source and said vessel, said excitation beam path comprising one or more excitation light fibers configured to transmit said excitation light to said vessel; a detector capable of detecting said emission light emitted from one of said plurality of luminescence labels; a detection arrangement configured to propagate said emission light along an emission beam path positioned between said vessel and said detector, said emission beam path comprising one or more emission light fibers configured to transmit said emission light to said detector; a filter wheel rotatably mounted to a wheel hub, thereby defining a spin axis, said filter wheel comprising a plurality of filter portion pairs positioned around said wheel hub, each pair relating to one of said plurality of luminescence labels and comprising a first filter portion adapted to filter wavelengths of said excitation light and transmit said excitation light for exciting the related luminescence label, and a second filter portion adapted to filter wavelengths of said emission light and transmit said emission light emitted by said related luminescence label, said first filter portion of one of said plurality of pairs comprising said second filter portion of another of said plurality of pairs, said plurality of filter portion pairs being positioned around said wheel hub such that, upon rotation of the filter wheel about the spin axis, one of said plurality of pairs can be brought into an operative orientation relative to said excitation and emission beam paths such that said first filter portion is positioned in said excitation beam path and said second filter portion is positioned in said emission beam path.