Biofuel production by recombinant microorganisms

摘要

Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate.

主权项

1. A recombinant prokaryote comprising:
(a) an α-isopropylmalate synthase polypeptide having at least 90% identity to SEQ ID NO:50 and having α-isopropylmalate synthase activity; (b) a β-isopropylmalate dehydrogenase polypeptide having at least 90% identity to SEQ ID NO:52 and having β-isopropylmalate dehydrogenase activity; (c) an α-isopropylmalate isomerase polypeptide having at least 90% identity to SEQ ID NO:54; (d) an α-isopropylmalate isomerase polypeptide having at least 90% identity to SEQ ID NO:56 and having α-isopropylmalate isomerase activity in combination with the α-isopropylmalate isomerase polypeptide of (c); (e) a 2-keto-acid decarboxylase polypeptide having at least 90% identity to SEQ ID NO:28 and having 2-keto acid decarboxylase activity; (f) a NADH-dependent alcohol dehydrogenase having at least 90% identity to SEQ ID NO:38; (g) an acetohydroxy acid synthase polypeptide having at least 90% identity to SEQ ID NO:42; (h) an acetohydroxy acid synthase polypeptide having at least 90% identity to SEQ ID NO:40 and having acetohydroxy acid synthase activity in combination with the acetohydroxy acid synthase polypeptide of (g); (i) an acetohydroxy acid isomeroreductase polypeptide having at least 90% identity to SEQ ID NO:44 and having acetohydroxy acid isomeroreductase activity; and (j) a dihydroxy-acid dehydratase polypeptide having at least 90% identity to SEQ ID NO:46 and having dihydroxy-acid dehydratase activity; and wherein the recombinant prokaryote produces 3-methyl-1-butanol.