| 摘要 |
Solution to problem The present invention provides an analysis method for a glucagon-secretin family peptide in a sample, the method comprising:
a cleavage step of a peptide bond of aspartic acid of a glucagon-secretin family peptide in a sample to obtain peptide fragment;
a separation/purification step of separating and purifying the peptide fragments cleaved in the cleavage step using liquid chromatography to elect a peptide fragment, a substance to be measured, on an N-terminal glucagon-secretin family peptide; and
an analysis step of carrying out mass spectrometry to the peptide fragments separated and purified in the separation/purification step to detect the peptide fragment on the N-terminal glucagon-secretin family peptide. |
