Method of using neutrilized DNA (N-DNA) as surface probe for high throughput detection platform

摘要

A method of using Neutrilized DNA (N-DNA) as a surface probe for a high throughput detection platform is disclosed. FET and SPRi are used as high throughput detection platforms to demonstrate that the N-DNA surface probe produces good results and enhances detection sensitivity. The N-DNA modifies the charged oxygen ions (O−) on the phosphate backbone through methylation, ethylation, propylation, or alkylation, so that the backbone is not charged after this modification to increase the hybridization efficiency, sensitivity and to make the signal more clear.

主权项

1. A high throughput detection platform comprising a neutralized DNA (N-DNA) probe of formula (I): wherein n is 1, 2, or 3 and each A, T, G, and C is any of adenine, thymine, guanine, and cytosine; wherein said probe can hybridize to a complementary target nucleic that is to be detected; and wherein said probe is covalently attached to a solid surface, said solid surface is that of a silicon nanowire field effect transistor (SiNW-FET).