LIPID PROBES AND USES THEREOF

摘要

Disclosed herein are methods, compositions, probes, assays and kits for identifying a lipid binding protein as a drug binding target. Also disclosed herein are methods, compositions, and probes for mapping a ligand binding site on a lipid binding protein, identification of lipid binding proteins, generating drug-lipid binding protein profiles, high throughput drug screening, and identification of drugs as potential lipid binding protein ligands.

主权项

1. A method of identifying a lipid binding protein as a drug binding target, comprising:
a. harvesting a set of lipid probe-protein complexes from a sample wherein the lipid probe comprises a lipid, a photoreactive group, and an affinity handle; b. analyzing the set of lipid probe-protein complexes by a proteomic analysis means; c. based on step b), assigning a value to each of the lipid binding proteins from the set of lipid probe-protein complexes; d. based on the value assigned in c), identifying a lipid binding protein as a drug binding target.