发明名称 |
Improved capsule chemistry analytical methods employing dioxetane chemiluminescence |
摘要 |
<p>Improved assay methods utilizing stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility are provided. Assays are performed by a capsule chemistry analytical assay method wherein fluid capsules comprising discrete aqueous segments containing either a chemiluminescent dioxetane reagent or an activating agent separated by an oil-based isolating fluid are flowed through a conduit, and subsequently reacted to produce light. The improvement comprises using a dioxetane substituted with two or more water-solubilizing groups disposed on the dioxetane structure to provide the chemiluminescence. Compositions comprising such a dioxetane, a non-polymeric surfactant enhancer and optionally a fluorescer, provide enhanced chemiluminescence and eliminate the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. <IMAGE></p> |
申请公布号 |
EP0757248(B1) |
申请公布日期 |
2001.05.23 |
申请号 |
EP19960112385 |
申请日期 |
1996.07.31 |
申请人 |
BOARD OF GOVERNORS;BAYER CORPORATION |
发明人 |
ADOLFSEN, ROBERT H.;AKHAVAN-TAFTI, HASHEM;DE SILVA, RENUKA;SCHAAP, PAUL A. |
分类号 |
G01N21/78;C07D321/00;C07F9/54;C09K11/06;C09K11/07;C12Q1/42;G01N33/53;G01N33/58;(IPC1-7):G01N33/52 |
主分类号 |
G01N21/78 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|