| 摘要 |
A reliable, fast and convenient method is provided to determine the chang e in concentration of enzyme in time in a biological medium in the presence of a signal substrate that corrects for substrate consumption, color-depende ncy of the signal and non-linearity between the concentration of the leaving group of the signal substrate and the amount of signal. The method comprise s the measurement of a calibrator curve in a suitable medium, such as a buff er, to determine the characteristics of the measured curve. These characteri stics will then allow to obtain this whole curve or a sufficient part thereo f again through a mathematical procedure based on a single-point measurement in a sample of a medium in which the enzyme generation takes place. This ha s the great advantage that there is no need to measure the whole calibrator curve in each individual medium, since a single-point measurement is adequat e.
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