Human skin equivalents comprising near diploid immortalized keratinocyte cells (NIKS)

摘要

Disclosed is a composition comprising a pre-graft human skin equivalent, said skin equivalent having a surface electrical capacitance of from about 40 to about 240 pF measured as the difference in reading over a 10 second interval, said skin equivalent having a combined content of ceramides 5, 6, and 7 in said skin equivalent of from about 20 to about 50% of total ceramide content, wherein said pre-graft skin equivalent comprises Near Diploid Immortalized Keratinocyte cells, and a method of screening for cellular toxicity using said skin equivalent.