发明名称 |
bactérias com genoma reduzido |
摘要 |
The present invention provides a bacterium having a genome that is genetically engineered to be at least 2 to 14% smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence. |
申请公布号 |
BRPI0307125(A2) |
申请公布日期 |
2016.11.01 |
申请号 |
BR20030307125 |
申请日期 |
2003.01.22 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION |
发明人 |
CHRISTOPHER D, HERRING;FREDERICK R. BLATTNER;GYORGY POSFAI;GUY PLUNKETT;JEREMY D. GLASNER;TREVOR MARTIN TWOSE |
分类号 |
C12N15/09;C12N15/10;C12N1/08;C12N1/20;C12N1/21;C12N15/70;C12N15/90;C12P21/02;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|