发明名称 |
METHOD AND DEVICE FOR DETERMINING NUCLEOTIDE SEQUENCE OF DNA |
摘要 |
A method for determining the nucleotide sequence of DNA includes forming of a pattern of oligonucleotides, its hybridization with the marked DNA to be tested, washing out under the conditions of dissociation of duplexes, identification of single substitutions of bases in the tested DNA by analysing the distribution of the mark and, depending on the results of the analysis, reconstruction of the nucleotide sequence of the tested DNA. The pattern of oligonucleotides is formed with their concentrations providing for the desired temperature of dissociation of duplexes in the course of washing. A device for determining the nucleotide sequence of DNA comprises a substrate (1) and a matrix (2) secured to the latter by means of a gel layer of a thickness not exceeding 30 mkm and containing a pattern of oligonucleotides of desired length. |
申请公布号 |
WO9216655(A1) |
申请公布日期 |
1992.10.01 |
申请号 |
WO1992RU00052 |
申请日期 |
1992.03.18 |
申请人 |
INSTITUT MOLEKULYARNOI BIOLOGII IMENI V.A.ENGELGAR |
发明人 |
KHRAPKO, KONSTANTIN RADIEVICH;KHORLIN, ALEXANDR ANATOLIEVICH;IVANOV, IGOR BORISOVICH;ERSHOV, GENNADY MOISEEVICH;LYSOV, JURY PETROVICH;FLORENTIEV, VLADIMIR LEONIDOVICH;MIRZABEKOV, ANDREI DARIEVICH |
分类号 |
C12Q1/68;B01J19/00;C12N15/00;C40B40/06;G01N33/50;G01N33/53;G01N37/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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