BIOLOGIC SAMPLE COLLECTION DEVICES AND METHODS OF PRODUCTION AND USE THEREOF

摘要

Collection devices and kits for biological sample collection include a biologic sample collection device having a hydrophilic swab matrix that includes a modified polycaprolactone (PCL). Methods of production and use thereof are also described herein. The biologic sample collection devices, kits and methods described herein are used to collect a biologic sample (e.g., blood, buccal cells, etc.) and to enable extraction of nucleic acids (e.g., DNA) from that biologic sample so that the nucleic acids can be analyzed (e.g., sequencing and subsequent analyses of DNA).

主权项

1. A method of extracting nucleic acids from a biologic sample collected by a biologic sample collection device comprising the steps of:
contacting the biologic sample and the biologic sample collection device comprising soluble and hydrophilic polycaprolactone coupled to a carrier, wherein at least a portion of the polycaprolactone solubilizes when exposed to a nucleic acid extraction reagent, with at least one nucleic acid extraction solution under conditions such that the polycaprolactone is solubilized or dissolved, resulting in a solution comprising nucleic acids separated from the biologic sample collection device; and extracting the nucleic acids from the solution.