发明名称 |
Purification of lipstatin |
摘要 |
The present invention relates to a method for the purification of the lipstatin. Impurities are removed from a lipstatin preparation by: a) mixing the preparation with a polar phase and to form a first two phase system in which the ratio of the lipstatin to the non-polar impurities in the polar phase is greater than in the preparation, and separating the phases from the first two phase system and collecting the lipstatin-enriched polar phase, and b) i) diluting the lipstatin-enriched polar phase from step a) with water to form a diluted lipstatin-enriched polar phase containing the polar solvent in a percentage of at least seventy percent of the polar phase and lower than in the lipstatin-enriched polar phase from step a), and mixing the diluted lipstatin-enriched polar phase with a second non-polar phase, the second non-polar phase being the same non-polar solvent as the first non-polar phase in the same or greater relative amount to the polar phase as in the first two phase system; or ii) mixing the lipstatin-enriched polar phase from step a) with a second non-polar phase, the second non-polar phase being the same non-polar solvent as the first non-polar phase in a greater relative amount to the polar phase as in the first two phase system; to form a second two phase system in which the ratio of the lipstatin to the polar impurities in the second non-polar phase is greater than in the lipid-enriched polar phase from step a), and separating the phases from the second two phase system and collecting the lipstatin-enriched second non-polar phase.
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申请公布号 |
US6156911(A) |
申请公布日期 |
2000.12.05 |
申请号 |
US20000491557 |
申请日期 |
2000.01.26 |
申请人 |
HOFFMANN-LA ROCHE INC. |
发明人 |
DOSWALD, STEPHAN;KUPFER, ERNST;STEINBAUER, GERHARD;STEINWENDER, ERICH |
分类号 |
C07D305/12;(IPC1-7):C07D305/12 |
主分类号 |
C07D305/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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