| 发明名称 |
METHOD TO IDENTIFY AND ISOLATE PLURIPOTENT STEM CELLS USING ENDOGENOUS BLUE FLUORESCENCE |
| 摘要 |
The instant disclosure provides for a method of identifying and isolating pluripotent stem cells and distinguishing pluripotent stem cells from differentiating/differentiated cells, using the property of endogenous blue fluorescence emitted from intracellular lipid bodies which serves as an endogenous marker for the pluripotent state. |
| 申请公布号 |
US2016305885(A1) |
申请公布日期 |
2016.10.20 |
| 申请号 |
US201314442226 |
申请日期 |
2013.11.12 |
| 申请人 |
NATIONAL CENTRE FOR BIOLOGICAL SCIENCES |
发明人 |
PANICKER Mitradas M.;MENON Radhika;THANGASELVAM Muthusamy;MUKHERJEE Odity |
| 分类号 |
G01N21/64;C12N5/0735;C12Q1/04 |
主分类号 |
G01N21/64 |
| 代理机构 |
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代理人 |
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| 主权项 |
1. A method for identifying pluripotent stem cell in a culture and optionally isolating the pluripotent stem cell from the culture, said method comprising acts of:
a) subjecting the culture to excitation at wavelength ranging from about 275 nm to about 410 nm for obtaining endogenous blue fluorescence emission from lipid body present within the pluripotent stem cell; and b) measuring intensity of the emission for identifying the pluripotent stem cell in the culture; c) optionally sorting the culture for isolating the pluripotent stem cell from the culture. |
| 地址 |
Karnataka IN |
