| 主权项 |
1. A fusion protein comprising a protein or fragment thereof encoded by a cell totipotency-related gene, and a transcriptional control domain or fragment thereof having transcriptional control activity which links directly to or links via a linker sequence to the protein or fragment thereof encoded by the cell totipotency-related gene;
preferably, the linker sequence is GGGGS; preferably, the protein or fragment thereof encoded by the cell totipotency-related gene links to the transcriptional control domain or fragment thereof having transcriptional control activity via amino terminal or carboxyl terminal; preferably, the cell totipotency-related gene is one or more selected from OCT4, SOX2, NANOG, SOX1, SOX3, SOX15, SOX18, STAT3, SMAD1, Sal4, Nr5a2, Dax1, Esrrb, Utf1, MyoD, CEBPα, Pax5, Pdx1, Ngn3, MafA, Ascl1, Brn2, Myt1l, Gata4, Mef2c and Tbx5; more preferably, the cell totipotency-related gene is one or more selected from OCT4, SOX2, NANOG, MyoD, CEBPα, Pax5, Pdx1, Ngn3, MafA, Ascl1, Brn2, Gata4, Mef2c and Tbx5; further preferably, the cell totipotency-related gene is one or more, such as one, two or three, selected from OCT4, SOX2 and NANOG; preferably, the transcriptional control domain is a transcriptional activation domain of mammal YAP protein or fragment thereof having transcriptional control activity; more preferably, the transcriptional control domain is a transcriptional activation domain of mouse, swine, caprine, bovine or human YAP protein or fragment thereof having transcriptional control activity; further preferably, the transcriptional control domain is a transcriptional activation domain of mouse YAP protein or fragment thereof having transcriptional control activity; further more preferably, the amino acid sequence of the transcriptional control domain is set forth in SEQ ID NO: 10. |
