Live bioload detection using microparticles

摘要

The present invention provides methods to concentrate cells onto microparticles, to concentrate the microparticles, and to detect the cells. The present invention also includes unitary sample preparation and detection devices to be used in accordance with the methods.

主权项

1. A unitary sample preparation and detection device, comprising:
a housing comprising at least two receptacles with a passageway therebetween; wherein an upper receptacle of said at least two receptacles comprises an opening configured to receive a sample and contains a cell concentration agent disposed therein; wherein a lower receptacle of said at least two receptacles includes a detection reagent disposed therein and a hydrogel comprising a cell extractant; means for isolating the upper receptacle from the lower receptacle; and means for transferring the cell concentration agent from the upper receptacle to the lower receptacle.