Method of making an avian cell line

摘要

The present invention relates to a method for producing avian cell lines, comprising gradual or complete withdrawal of growth factors, serum and/or feeder layer so that the established lines are adherent or nonadherent cells capable of proliferating indefinitely in a basic culture medium. The invention also relates to the cells derived from such lines which are particularly useful for the production of substances of interest.

主权项

1. A method for establishing an avian cell line, said method comprising:
(a) isolating cells from a blastodermal disk of a fertilized avian egg; (b) culturing said isolated cells in a basal culture medium supplemented with
i) mouse stem cell factor (SCF), insulin-like growth factor 1 (IGF-1), ciliary neurotrophic factor (CNTF), interleukin 6 (IL-6), soluble IL-6 receptor (sIL-6r), and basic fibroblast growth factor (bFGF), and optionally interleukin 11 (IL- 11);(ii) a layer of feeder cells; and(iii) animal serum; (c) culturing said cells of step (b) in basal culture medium comprising IGF-1 and CNTF as the only supplemental growth factors and cytokines, a layer of feeder cells, and animal serum such that an avian cell line capable of being infected by and replicating viruses is obtained.