Chromatographic system and method of isolating compound in sample using chromatographic system

摘要

A method is provided for isolating a compound in a sample by chromatography. The method includes determining an analytical gradient of an analytical system; performing an analytical run using the analytical gradient; identifying a target compound in the sample and determining an analytical retention time of the target compound using chromatographic results of the analytical run; determining an elution point of the target compound using the analytical retention time, the analytical gradient and characteristics of the analytical system; determining automatically a focusing gradient of a preparative system, including a slope segment; and performing a preparative run using the focusing gradient to separate the target compound from the sample. The slope segment includes a slope determining a concentration of solvent in a solvent mixture, which linearly increases from a first offset below the elution point of the target compound to a second offset above the elution point of the target compound.

主权项

1. A method of isolating a compound in a sample, comprising a plurality of compounds, by chromatography, the method comprising:
determining an analytical gradient of an analytical system; performing an analytical run using the analytical gradient; identifying a target compound in the sample using chromatographic results of the analytical run; determining an analytical retention time of the target compound using the chromatographic results of the analytical run; determining an elution point of the target compound using the analytical retention time, the analytical gradient and characteristics of the analytical system; determining automatically a focusing gradient of a preparative system, up-scaled from the analytical gradient, the focusing gradient including a slope segment; and performing a preparative run using the focusing gradient to separate the target compound from the sample, wherein the slope segment of the focusing gradient includes a slope determining a concentration of solvent in a solvent mixture, the concentration linearly increasing from a first offset below the elution point of the target compound to a second offset above the elution point of the target compound.