发明名称 |
RECOMBINANT NUCLEOTIDE-SPECIFIC RIBONUCLEASE AND METHOD OF PRODUCING AND USING SAME |
摘要 |
A recombinant ribonuclease is disclosed. The recombinant ribonuclease is produced by introducing a recombinant DNA sequence into a host; activating expression of the recombinant DNA sequence within the host to produce the recombinant ribonuclease; and isolating the recombinant ribonuclease from the host. Additionally, a method of analyzing an RNA sequence includes digesting the RNA with a first recombinant ribonuclease to give digestion products comprising nucleotides of the RNA sequence; and analyzing the digestion products using an analytical method to provide the identity of at least some of the nucleotides. The recombinant ribonuclease includes at least one of a uridine-specific recombinant RNase MCI and a cytidine-specific recombinant RNase Cusativin. |
申请公布号 |
WO2016172678(A3) |
申请公布日期 |
2016.12.15 |
申请号 |
WO2016US29151 |
申请日期 |
2016.04.25 |
申请人 |
UNIVERSITY OF CINCINNATI |
发明人 |
ADDEPALLI, Balasubrahmanyam;LESSNER, Nicholas, Paul;LIMBACH, Patrick, Alan;VENUS, Sarah |
分类号 |
C12N9/22;C07H21/04;C07K14/00;C12P21/06;C12Q1/68 |
主分类号 |
C12N9/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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