发明名称 |
METHODS FOR REFOLDING PROTEINS CONTAINING FREE CYSTEINE RESIDUES |
摘要 |
The present invention relates to novel methods for making and refolding insoluble or aggregated proteins having free cysteines in which a host cell expressing the protein is exposed to a cysteine blocking agent. The soluble, refolded proteins produced by the novel methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form PEGylated proteins. |
申请公布号 |
EP1284987(B1) |
申请公布日期 |
2007.07.18 |
申请号 |
EP20010941504 |
申请日期 |
2001.05.16 |
申请人 |
BOLDER BIOTECHNOLOGY, INC. |
发明人 |
ROSENDAHL, MARY, S.;COX, GEORGE, N.;DOHERTY, DANIEL, H. |
分类号 |
C07K1/113;C12N15/09;C07K1/02;C07K14/00;C07K14/505;C07K14/515;C07K14/52;C07K14/535;C07K14/56;C07K14/61;C07K14/78;C12P21/02 |
主分类号 |
C07K1/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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