| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method and a composition for differentiating stromal cells from adipose tissue into cells having osteoblastic properties, and a method for improving a subject's bone structure. <P>SOLUTION: The method comprises a process for culturing the stromal cells from the adipose tissue in β-glycerophosphate and ascorbic acid and/or ascorbate-2-phosphate for a time sufficient to allow differentiation of the cells into osteoblasts. The method and the composition like this are useful for producing the osteoblasts for autologous transplantation into a bone at a surgical site or injury. This composition comprises adipose stromal cells, a medium able to support the growth of fibroblasts and sufficient amounts of β-glycerophosphate and ascorbic acid and/or ascorbate-2-phosphate or both of them to induce the differentiation of the stromal cells into osteoblasts. Further, a method for identifying compounds affecting the differentiation of osteoblasts is provided. The compound is useful in the study of bone generation and treatment of bone disorders including bone fractures and osteoporosis. <P>COPYRIGHT: (C)2010,JPO&INPIT |
