Process for detecting cells from a sample

摘要

A process for detecting cells involves applying a liquid, cell-containing sample to a porous support so that the cells enter the support pores and are retained by a cell-specific binding molecule on the pore surface and, optionally after the cells are detectably labeled, performing an optical read-out by high density imaging the entire porous support volume using an optical system having an optical axis that runs in the direction of sample flow.

主权项

1. A process for detecting cells from a sample, comprising the following steps:
(a) applying the sample or a fraction of the sample in liquid phase in a direction of flow to a porous two-dimensional support having an entire volume with pores having on the surface thereof at least one binding molecule specific for the cells to be detected or for fragments thereof; whereby the cells to be detected or fragments thereof enter into the pores of said porous support, said pores having a mean pore size that allow entry of the cells or fragments thereof into the pores, whereby the cells or fragments to be detected are retained in the entire support volume by binding to the at least one binding molecule specific for the cells or fragments to be detected; and (b) performing an optical read-out by high definition imaging the entire porous support volume using an optical system having an optical axis, wherein the optical axis of the optical system runs in the direction of flow.