Sporulation-deficient thermophilic microorganisms for the production of ethanol

摘要

A thermophilic microorganism comprising a modification that prevents sporulation, wherein the modification inactivates the native spo0A gene.

主权项

1. An isolated thermophilic microorganism comprising a modification that decreases sporulation compared with wild-type, wherein the modification inactivates the native stage 0 sporulation protein A (spo0A) gene, wherein the microorganism further comprises a modification that inactivates the native lactate dehydrogenase (ldh) gene, wherein the microorganism is a Geobacillus species, and wherein the modification of the native spo0A gene increases the microorganism's ethanol tolerance and rate of ethanol production relative to the microorganism without the modification of the spo0A gene.