Flavin-bound glucose dehydrogenases, a method for producing a flavin-bound glucose dehydrogenase, and yeast transformant used for the same

摘要

A heat-resistant flavin-bound glucose dehydrogenase having a high substrate specificity for D-glucose, an method for producing the same, and a transformant used for the same. A flavin-bound glucose dehydrogenase gene encoding a flavin-bound glucose dehydrogenase derived from Mucor is introduced into yeast, Zygosaccharomyces, to obtain a transformant. Subsequently, the yeast transformant is cultured to obtain a flavin-bound glucose dehydrogenase from the culture. The heat-resistant flavin-bound glucose dehydrogenase is less susceptible to the effects of dissolved oxygen and allows accurate measurement of glucose even in the presence of sugar compounds other than glucose in a sample.

主权项

1. A flavin-bound glucose dehydrogenase having the amino acid sequence of SEQ ID NO: 3, and having the following properties (i) to (iv):
(i) action: glucose dehydrogenase activity in the presence of an electron acceptor; (ii) molecular weight: the molecular weight of the polypeptide chain portion of the protein is approximately 80 kDa; (iii) substrate specificity: lower reactivity to maltose, D-galactose, and D-xylose than that to D-glucose; and (iv) 50% or above residual activity after heat treatment at 50° C. for 15 minutes, wherein the flavin-bound glucose dehydrogenase is produced by a yeast transformant.