| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a method for suitably and quantitatively analyzing the gene expression of a single cell. <P>SOLUTION: This method for detecting nucleic acid is provided by comprising a process of collecting the single cell from a specimen containing at least the single cell, a process of lysing the cell for dissolving the cell membrane of the collected single cell to elute the nucleic acids of the cell, a DNA-decomposing process of decomposing the DNA from the eluted nucleic acids with a DNA-decomposing enzyme, a process of hybridizing mRNA among RNAs contained in the single cell with an oligo(dT) immobilized on a carrier, a process of performing a reverse transcription reaction on the mRNA hybridized with the oligo(dT) to prepare a single cell-derived cDNA-immobilized carrier library consisting of the single cell-derived cDNA immobilized on the carrier and a process of detecting the amplified amount while performing an amplification reaction of the cDNA immobilized on the carrier. <P>COPYRIGHT: (C)2008,JPO&INPIT</p> |
