| 摘要 |
This invention provides apparatus and methods that enable the direct measurement of an analyte sample in an assay well by forming an independent measuring region. The sample to be measured can be separated into portions, for example, a bound portion and a free portion. To form the independent measuring region, a layer-forming material that can restrict the passage of radiant energy that is measured by a detection device is added to the assay well. The layer-forming material forms a layer that restricts the passage of radiant energy from the region. Thus, a sample portion in one separate region may be measured by a detection device separately and independently of a portion of the sample in a different region. In one example, a second portion in another region can be separately and independently measured by a detection device without the removal of the portions from the assay well. |
