METHOD FOR PRODUCING A STANDARD FOR DETECTING PROTEIN AGGREGATES OF A PROTEIN MISFOLDING DISEASE, AS WELL AS A STANDARD AND THE USE THEREOF

摘要

The invention relates to a method for producing a standard for detecting protein aggregates of a protein misfolding disease, comprising the following steps: A) providing an inorganic nanoparticle with the size of the aggregate of the protein misfolding disease; B) forming free amino groups or free carboxyl groups on the surface of the nanoparticle (for functionalising the nanoparticle surface to form an amine- or carboxyl-functionalised nanoparticle); C) i) bonding maleinimide-spacer-carboxylic acid to the free amino groups in step B), or ii) converting the free carboxyl groups in step B) into NHS-esters; D) bonding monomers of the protein aggregate i) to the maleinimide-spacer-carboxylic acid via a sulfhydryl group at the free end of the monomers, or ii) to the NHS-esters via the amino group at the free end of the monomer. The invention also relates to a standard as well as the use thereof in the detection of protein aggregates occurring in the case of protein misfolding diseases.