| 摘要 |
A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region. |
| 主权项 |
1. A cartridge for processing a sample, the cartridge comprising:
a first layer comprising a sample port and a fluid port at a broad surface of the first layer; an intermediate substrate coupled to the first layer and partially separated from the first layer by a film layer, and configured to form a sealed waste chamber with a corrugated surface directly opposing the first layer, wherein the corrugated surface defines a void external to the waste chamber; and a fluidic pathway, superior to the intermediate substrate and at least partially separated from the corrugated surface of the waste chamber by an elastomeric layer, wherein the fluidic pathway is fluidly coupled to the sample port and the fluid port, and wherein, upon deformation of the elastomeric layer at an occlusion position of the fluidic pathway, by way of the void of the corrugated surface, the fluidic pathway is configured to transfer waste fluid through an opening of the intermediate substrate and into an interior portion of the corrugated surface of the waste chamber. |
