| 摘要 |
The present invention relates to chemistry and, more specifically, to gene engineering. Method for treatment of deoxyribonucleic acid sequence by restrictase, suitable for said treatment of practically any of said DNA sequence, comprises the following steps: binding co-valently a chemical compound capable to recognize specifically a concrete DNA site (component A) with a photoligand (component B) to obtain a new substance; adding the substance obtained to said DNA sequence to receive a complex; and illuminating by light the complex received with wave lengths selected from spectrum covered visible and ultraviolet ranges. The invention may be utilized for DNA site-specific restriction and site-specific mutagenesis in gene engineering, biotechnology, medicine, veterinary. |
