摘要 |
<P>PROBLEM TO BE SOLVED: To provide a cell culture medium and a cell culture system which eliminate or at least reduce the requirement for exogenous components such as serum and feeder cells. <P>SOLUTION: The cell culture medium comprises an IGF (Insulin-like Growth Factor), vitronectin or fibronectin, and optionally an IGFBP (Insulin-like Growth Factor Binding Protein), and is particularly suitable for propagating keratinocytes for subsequent use in skin growth and regeneration. Compositions and methods for skin growth and regeneration in situ, which utilize aerosol delivery of cultured keratinocytes, are also provided. <P>COPYRIGHT: (C)2010,JPO&INPIT |