| 摘要 |
<p>The present invention relates to a method for identification of disease-related peptides and/or proteins for use as markers in diagnosis, prognosis, or therapeutic monitoring of disease, said method comprising the steps of: (a) providing an optionally processed sample of a diseased body tissue or fluid as a test sample, and an optionally processed sample of a corresponding healthy body tissue or fluid as a reference sample, wherein said samples comprise peptides and/or proteins; (b) subjecting both test and reference sample to MALDI- FT-ICR mass spectrometry to generate mass spectra for individual peptides in each sample and to quantify the amount of individual peptides present in each sample; (c) comparing the amount of an individual peptide present in the test sample with the amount of a peptide having a corresponding mass spectrum in the reference sample to generate a list of peptides differentially expressed between test and reference sample, and (d) subjecting the test and/or reference sample of step (a) to tandem mass spectrometry (MS-MS), in order to identify the differentially expressed peptides and/or the proteins from which they derive thus providing a candidate marker protein or marker peptide.</p> |
