| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for judging malignant metastatic stomach cancer, a method for evaluating the risk of peritoneal metastasis and recurrence of the stomach cancer, a method for judging the effectiveness of an anticancer agent and a kit for them by elucidating the mechanism of the disseminating peritoneal metastasis of the stomach cancer and by identifying a protein becoming a target of a new treating agent or a tumor marker having a high specificity. SOLUTION: This method for judging malignant metastatic cancer is provided by comparing the two dimensional electrophoretic images of cell lysate of stomach cancer cell line MKN45 and its peritoneal disseminated metastatic cell line clone MKN45P by using a two-dimensional difference gel electrophoresis (2D-DIGE) method, and then picking up spots having the significantly different amounts of expressed proteins between the both cell lines by a statistical method, performing a mass spectrum analysis [MALDI-TOF (Matrix Assisted Laser Desorption Time of flight) MS: proteomics analyzer 4700] on protein at each of the spots and performing the search of data base. As a result, several kinds of proteins increase or decrease in the peritoneal disseminated metastatic cell line as compared with those of the stomach cancer cell line can be identified. COPYRIGHT: (C)2007,JPO&INPIT
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