| 主权项 |
1. A method of initiating a plurality of plant somatic embryos from a megagametophyte comprising the steps of:
(a) dissecting a megagametophyte transversally to the longitudinal axis into two parts at a position that is located proximal to the micropylar end, wherein the megagametophyte is dissected transversally to the longitudinal axis at a position that is from about one-eighth to about one-half the length of the megagametophyte and proximal to the micropylar end; and (b) culturing either or both parts of the dissected megagametophyte to initiate the formation of embryogenic suspensor mass,wherein the megagametophyte is dissected before a dominant embryo has formed in the megagametophyte. |
