Compositions for linking DNA-binding domains and cleavage domains

摘要

Disclosed herein are compositions for linking DNA binding domains and cleavage domains (or cleavage half-domains) to form non-naturally occurring nucleases. Also described are methods of making and using compositions comprising these linkers.

主权项

1. A fusion protein comprising from N-terminus to C-terminus:
a DNA-binding domain that binds to a nucleotide target site; a ZC linker (SEQ ID NO:2); and a FokI cleavage domain, wherein the FokI cleavage domain comprises the sequence shown in residues 158 to 356 of SEQ ID NO:3 or residues 158 to 360 of SEQ ID NO:4.