发明名称 |
Human lambda light chain mice |
摘要 |
Genetically modified mice are provided that express human λ variable (hVλ) sequences, including mice that express hVλ sequences from an endogenous mouse λ light chain locus, mice that express hVλ sequences from an endogenous mouse κ light chain locus, and mice that express hVλ sequences from a transgene or an episome wherein the hVλ sequence is linked to a mouse constant sequence. Mice are provided that are a source of somatically mutated human λ variable sequences useful for making antigen-binding proteins. Compositions and methods for making antigen-binding proteins that comprise human λ variable sequences, including human antibodies, are provided. |
申请公布号 |
US9394373(B2) |
申请公布日期 |
2016.07.19 |
申请号 |
US201414561867 |
申请日期 |
2014.12.05 |
申请人 |
Regeneron Pharmaceuticals, Inc. |
发明人 |
Macdonald Lynn;Stevens Sean;Gurer Cagan;Murphy Andrew J.;Meagher Karolina A. |
分类号 |
C12N15/01;C07K16/46;A01K67/027;C07K16/00;C12N15/85;C07K16/18;C12N15/10 |
主分类号 |
C12N15/01 |
代理机构 |
Foley Hoag LLP |
代理人 |
Jones Brendan T.;Miyamoto Neil;Foley Hoag LLP |
主权项 |
1. A transgenic mouse comprising in its genome an endogenous λ light chain immunoglobulin locus comprising a deletion of the endogenous mouse Vλ1-Jλ3-Cλ3-Jλ1-Cλ1 gene cluster and a deletion of a region spanning from Vλ2 to Jλ2 of the endogenous mouse Vλ2-Vλ3-Jλ2-Cλ2-Jλ4-Cλ4 gene cluster. |
地址 |
Tarrytown NY US |