Method for the synthesis of a bifunctional complex

摘要

Disclosed is a method for obtaining a bifunctional complex comprising a display molecule part and a coding part, wherein a nascent bifuntional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is reacted at the chemical reaction site with one or more reactants, and provided with respective tag(s) identifying the reactant(s) at the priming site is using one or more enzymes.

主权项

1. A method for synthesizing a library of bifunctional complexes comprising a reaction product and an identifier oligonucleotide, the method comprising:
performing a first synthesis round, comprising:
a) Adding a bridging molecule part to each of a plurality of reaction wells;b) Adding a chemical entity to each of the plurality of reaction wells;c) Adding an oligonucleotide tag to each of the plurality of reaction wells;d) Attaching the oligonucleotide tag to the chemical entity through the bridging molecule part in each of the plurality of reaction wells, wherein the oligonucleotide tag identifies the chemical entity attached thereto; performing a second synthesis round in each of the plurality of reaction wells, comprising
e) repeating steps b) through c), andf) reacting the chemical entity added in step (e) with the chemical entities attached in step (d) to provide the reaction products, and attaching the oligonucleotide tag added in step (e) to the oligonucleotide tags attached in step (d) to provide the identifier oligonucleotides, wherein the oligonucleotide identifier comprises at least two oligonucleotide tags, and the reaction product comprises at least two reacted chemical entities, each oligonucleotide tag identifying a reacted chemical entity;
g) optionally performing one or more additional synthesis rounds in each of the plurality of reaction wells by repeating steps (e) and (f); andcombining the contents of a plurality of reaction wells from step (f) or step (g) to provide the library of bifunctional complexes;the method further comprising,
contacting one or more bifunctional complexes with a complimentary oligonucleotide comprising anti-tags capable of hybridizing with at least two of the oligonucleotide tags of the identifier oligonucleotide of the one or more bifunctional complexes.