PROBES AND PRIMERS FOR THE RAPID AND RELIABLE QUANTIFICATION OF THE GENES SPECIFIC TO PROSTATE CANCER, USING SUITABLE REFERENCE GENES

摘要

The present invention relates to new sets of primers and probes and to the use thereof for detecting prostate-specific genes, i.e. the PCA3, TMPRSS2- ERG and AMACR genes, and reference genes selected from the RPL5, HPRT1 and HNRNPA1 genes for normalising the target gene expression analysis data from patient samples, whether these are tissue samples or biological fluids. The sets of primers and probes of the target genes and reference genes are more sensitive than those cited in the literature, and will be used in real-time PCR (qPCR) in simplex or multiplex format for a reliable, rapid and less costly quantification.