Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use

摘要

Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.

主权项

1. A chemical compound having the structure: wherein: n is a non-negative integer; X is oxygen or sulfur; R13, R14, R15, R16, R17, R18, R20, and R21 independently comprise hydrogen, a hydroxyl group, an alkoxy group, a thiol, a thioalkyl, a thioaryl, a halogen, an alkyl group, an alkenyl group, an alkynyl group, an aromatic group, a primary amine group, a secondary amine group, a tertiary amine group, a reactive group, or combinations thereof wherein one of R13, R14, R15, R16, R17 comprises an aromatic group and R19 is an aromatic or alkylaromatic group R22 is an alkyl group.