Standardizing fluorescence microscopy systems

摘要

Systems and methods for standardizing one or more fluorescence scanning instruments to a reference system by separating the effects of drift and normalization. In an embodiment, a drift image comprising an image of a drift reference slide is captured by a system to be standardized. A drift measurement is calculated using the drift image. A first normalization image comprising an image of a normalization slide is also captured by the system to be standardized. A reference normalization image, also comprising an image of the normalization slide, is captured by a reference system. The first normalization image is compared to the reference normalization image to determine a gamma value and offset value for the system to be standardized.

主权项

1. A method for standardizing a fluorescence microscopy imaging system, the method comprising:
by a first imaging system to be standardized, capturing a first normalization image comprising an image of a normalization slide; by a reference imaging system, capturing a reference normalization image comprising an image of the normalization slide; comparing the first normalization image to the reference normalization image; and determining a gamma value and offset value for the first imaging system based on the comparison.