Compositions and methods for preparing oligonucleotide solutions

摘要

The present invention is directed to methods and compositions for generating a pool of oligonucleotides. The invention finds use in preparing a population or subpopulations of oligonucleotides in solution. The pool of oligonucleotides finds use in a variety of nucleic acid detection and/or amplification assays.

主权项

1. A method of detecting the presence of target nucleic acids comprising:
a) obtaining a first substrate comprising greater than 50 different single-stranded oligonucleotides linked to discrete sites on the first substrate; b) releasing said greater than 50 different single stranded oligonucleotides from said first substrate as a pool of greater than 50 different single stranded oligonucleotides; c) contacting said pool of greater than 50 different single stranded oligonucleotides with a composition comprising target nucleic acids, whereby said target nucleic acids hybridize with greater than 50 different single stranded oligonucleotides in said pool of oligonucleotides; d) capturing said greater than 50 different single stranded oligonucleotides in said pool of oligonucleotides hybridized with said target nucleic acids on a second substrate; e) contacting said target nucleic acids with a primer, wherein said target nucleic acids are amplified upon binding to said primer; and f) detecting the presence of said target nucleic acids.