Bioreactor using acoustic standing waves

摘要

A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.

主权项

1. A system comprising:
a bioreactor including a reaction vessel having an internal volume, an agitator for circulating associated fluid containing cells and biomolecules within the internal volume, a feed inlet, and an outlet; and a filtering device, comprising:
an inlet fluidly connected to the bioreactor outlet for continuously receiving the associated fluid containing cells and biomolecules from the bioreactor;a flow chamber through which the associated fluid can flow;a sleeve surrounding the flow chamber, the sleeve including an ultrasonic transducer and a reflector located opposite the ultrasonic transducer, the ultrasonic transducer being driven to produce a multi-dimensional standing wave in the flow chamber that separates the biomolecules from the associated fluid;a product outlet through which the biomolecules are recovered; anda recycle outlet downstream of the flow chamber connected to a recycle inlet of the reaction vessel, for sending the associated fluid containing cells back to the bioreactor.