Instruments for biological sample-to-answer devices

摘要

Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers.

主权项

1. A portable instrument comprising:
a portable housing structure; a sample-to-answer device housing element, the sample-to-answer device housing element positioned and configured to attach to a sample-to-answer device, the sample to answer device having a reaction cartridge; a moving plate, positioned and directly adjacent to the sample-to-answer device housing element and configured to attach to the sample-to-answer device housing element; a solenoid, comprising a first end and a second end, the first end secured to the portable housing structure, the second end secured to the moving plate, wherein the solenoid is positioned and directly adjacent to the moving plate and configured to move the moving plate based on an input of a user to position the reaction cartridge in a correct position for optical interrogation of a target region of the reaction cartridge; at least one light source, configured to emit a first set of electromagnetic waves in a desired wavelength range; a waveguide element, attached directly below the at least one light source and configured to focus and direct the first set of electromagnetic waves onto the reaction cartridge of the sample-to-answer device housing element, such that a second set electromagnetic waves, which is resultant from the first set electromagnetic waves hitting the target region of the reaction cartridge, is configured to be emitted from the target region of the reaction cartridge, said waveguide element configured to irradiate a prism of the reaction cartridge of the sample-to-answer device; at least one detector, positioned and directly adjacent to the waveguide element and the light source and configured to receive the second set of electromagnetic waves from a reaction chamber of the reaction cartridge; and a printed circuit board directly fixed to the portable housing structure, comprising electronic components configured to control the operation of the solenoid and thereby configured to operate the moving plate, configured to activate the at least one light source, and configured to receive data from the at least one detector, based on the input from the user wherein the at least one light source, the waveguide element, and the at least one detector are each directly fixed to the portable housing structure and positioned adjacent the sample-to-answer device housing element so as together form an integrated instrument.