| 摘要 |
(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date W O 2013/126793 A2 29 August 2013 (29.08.2013) W I PO I P CT (51) International Patent Classification: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, C12Q 1/68 (2006.01) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, (21) International Application Number: HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, PCT/US2013/027457 KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, (22) International Filing Date: ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, 22 February 2013 (22.02.2013) NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, (25) Filing Language: English TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, (26) Publication Language: English ZM, ZW. (30) Priority Data: (84) Designated States (unless otherwise indicated, for every 61/603,091 24 February 2012 (24.02.2012) US kind of regional protection available): ARIPO (BW, GH, 61/725,401 12 November 2012 (12.11.2012) US GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (71) Applicant: GEN-PROBE PRODESSE, INC. [US/US]; TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, Gen-Probe Prodesse, Inc., 10210 Genetic Center Drive, EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, San Diego, California 92121-4362 (US). MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (72) Inventor: TYLER, Ejan; c/o GEN-PROBE INCORPOR- TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ATED, 10210 Genetic Center Drive, San Diego, California ML, MR, NE, SN, TD, TG). 92121-4362 (US). Published: (74) Agent: LANDES, Jeffrey E.; Gen-Probe Incorporated, - without international search report and to be republished 10210 Genetic Center Drive, San Diego, California 92121- upon receipt of that report (Rule 48.2(g)) 4362 (US). - with sequence listing part of description (Rule 5.2(a)) (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, (54) Title: DETECTION OF SHIGA TOXIN GENES IN BACTERIA (57) Abstract: The disclosed invention is related to methods, compositions and kits for targeting nucleic acid derived from Shiga toxin-producing bacteria such as E. coli. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one pair of amplification oligomers. |
