| 摘要 |
The present disclosure provides a composition with ultra sensitivity for detection of nucleic acid and the method of use thereof. The composition comprises a target probe capable of hybridizing to a target nucleic acid, at least one first bridge probe, at least one second bridge probe, and a label probe. The target probe includes a pre-bridge region having a first tail nucleotide sequence. The first bridge probe includes sequentially a first head region having a first head nucleotide sequence, a first gap region having a gap nucleotide sequence, and a first tail region having the first tail nucleotide sequence. The second bridge probe includes sequentially a second head region having a second head nucleotide sequence complementary to the first head nucleotide sequence, a second gap region having the gap nucleotide sequence, and a second tail region having a second tail nucleotide sequence complementary to the first tail nucleotide sequence. The label probe is capable of hybridizing to the first and the second gap nucleotide region. |
