发明名称 |
Permuted and nonpermuted luciferase biosensors binding cyclic nucleotides |
摘要 |
A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. |
申请公布号 |
EP2004813(A2) |
申请公布日期 |
2008.12.24 |
申请号 |
EP20070754666 |
申请日期 |
2007.04.02 |
申请人 |
PROMEGA CORPORATION |
发明人 |
BINKOWSKI, BROCK;FAN, FRANK;WIGDAL, SUSAN;WOOD, KEITH, V.;WOOD, MONIKA, G. |
分类号 |
C12N9/02;C12N15/53;C12Q1/66;G01N33/573 |
主分类号 |
C12N9/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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