METHOD FOR TREATING PANCREATITIS WITH MESENCHYMAL STEM CELLS

摘要

The present application discloses a method of treating pancreatitis by administering to the patient mesenchymal stem cells that are obtained by manipulating a biological sample of cells, which includes multi-lineage stem cells, progenitor cells, other marrow stromal cells: allowing the sample of cells to settle in a container; transferring supernatant from the container to another container; and isolating cells from the supernatant, which has comparatively lower density in the sample.

主权项

1. A method of treating pancreatitis, or reducing pancreatic edema or reducing relative weight of pancreas or increasing population of acinar cells or preventing necrosis of acinar cells of a subject suffering from pancreatitis, comprising:
(i) obtaining a biological sample of bone marrow, peripheral blood, cord blood, fatty tissue sample, or cytokine-activated peripheral blood cells; (ii) allowing the biological sample of bone marrow, peripheral blood, cord blood, fatty tissue sample, or cytokine-activated peripheral blood cells to settle in a container; (iii) transferring supernatant which contains comparatively less dense cells in the sample compared to other cells from the container to another container in a serial manner at least two times; (iv) isolating less dense cells from the supernatant; and (v) administering the cells obtained in step (iv) to a subject suffering from pancreatitis, wherein the bone marrow, peripheral blood, cord blood, fatty tissue sample, or cytokine-activated peripheral blood cells do not undergo centrifugation of greater than 1,000 rpm in steps (i) to (iii).