Detecting MicroRNA and Iso-MicroRNA to Evaluate Vascular and Cardiac Health

摘要

This disclosure relates to diagnosing or evaluating the state of vascular or cardiac health of a subject by detecting alterations in the microRNA and/or iso-microRNA profile contained in a sample derived from the subject. Typically, the sample is derived from blood such as a plasma sample or portions further isolated therefrom. In certain embodiments, the sample is plasma from which microvesicles have been removed or the sample is enriched with blood derived microvesicles.

主权项

1. A method for diagnosing a subject as being at risk or having cardiovascular disease comprising measuring in a sample the presence of one or more of the following iso-microRNA (iso-miR) selected from:iso-miR-10b,(SEQ ID NO: 1)UACCCUGUAGAACCGAAUUUGU,wherein the 3′ U is not followed by a G;iso-miR-30d(SEQ ID NO: 2)UGUAAACAUCCCCGACUGGAAGCU;iso-miR-93(SEQ ID NO: 3)CAAAGUGCUGUUCGUGCAGGUwherein, the 3′ U is not followed by a AG;iso-miR-143(SEQ ID NO: 4)UGAGAUGAAGCACUGUAGCU,wherein, the 3′ U is not followed by a C;iso-miR-181a(SEQ ID NO: 5)AACAUUCAACGCUGUCGGUGAGwherein, the 3′ G is not followed by a U;iso-miR-182(SEQ ID NO: 6)UUUGGCAAUGGUAGAACUCACA,wherein, the 3′ A is not followed by a CU;iso-miR-744(SEQ ID NO: 7)UGCGGGGCUAGGGCUAACAGC,wherein, the 3′ C is not followed by a A; and correlating an increase or decrease in the amount of iso-miR compared to a normal or reference value as being at risk or having cardiovascular disease.